Study Report
Basic Info
Reference |
Lu AT, 200818213623
|
Citation |
Lu A. T., Ogdie M. N., Jarvelin M. R., Moilanen I. K., Loo S. K., McCracken J. T., McGough J. J., Yang M. H., Peltonen L., Nelson S. F., Cantor R. M. and Smalley S. L. (2008) "Association of the cannabinoid receptor gene (CNR1) with ADHD and post-traumatic stress disorder." Am J Med Genet B Neuropsychiatr Genet, 147B(8): 1488-94.
|
Study Design |
case-control and family-based |
Study Type |
Candidate-gene association study |
Sample Size |
187 trios; 159 cases and 151 controls |
Predominant Ethnicity |
Caucasian |
Population |
USA, Finland |
Gender |
130 (69.5%) males and 57 (30.5%) females among 187 children in the family-based sample; 201 (64.8%) males and 109 (35.2%) females in the case-control sample |
Age Group |
Children/Adolescents and Adults
:
mean age 12.7, SD=3.3 years (range, 5-25) in the family-based sample; mean age 16.1,SD=0.3 years in the case-control sample
|
Detail Info
Summary |
They tested SNP variants at the CNR1 gene in two independent samples-an unselected adolescent sample from Northern Finland, and a family-based sample of trios (an ADHD child and their parents). In addition to using the trios for association study, the parents (with and without ADHD) were used as an additional case/control sample of adults for association tests. ADHD and its co-morbid psychiatric disorders were examined. A significant association was detected for a SNP haplotype (C-G) with ADHD (P-value=0.008). A sex by genotype interaction was observed as well with this haplotype posing a greater risk in males than females. |
Total Sample |
a sample of 187 trios randomly drawn from the affected sibling pair sample of the UCLA ADHD Genetic study were available to apply family-based association tests; the second independent sample consists of adolescent ADHD cases (n=159) and controls (n=151) drawn from a Northern Finnish Birth Cohort |
Sample Collection |
81.8% are Caucasians, 3.2% Hispanic, 4.8% are other ethnic groupings, and 10.2% are mixed-ethnic group in the family-based sample, Northern Finnish Birth Cohort in the case-control sample |
Diagnosis Description |
A similar diagnostic system is in place for samples used in the current analyses. ADHD and other psychiatric diagnoses were determined using a semi-structured psychiatric interview, KSADS-PL for children 5-18, and SADS-LAR for adults with the Behavioral Disorders section of the KSADS-PL used to determine lifetime and current ADHD, oppositional defiant disorder and conduct disorder. |
Technique |
Genotyping was performed using TaqMan SNP Genotyping Assays and the 7900HT Fast Real-Time PCR System for fluorescent read detection and allelic clustering. All assays were performed in 5 ml volume in 384-well plates according to the manufacturers' specifications. |
Analysis Method |
Associations of SNPs and haplotypes with the psychiatric diagnostic phenotypes under analysis were assessed using FBAT for LA parent-child trios or a case-control test for the Finnish sample and Caucasian parents in the LA trios. |
Result Description |
A significant association was detected for a SNP haplotype with ADHD. A sex by genotype interaction was observed as well with this haplotype posing a greater risk in males than females. An association of an alternative SNP haplotype in this gene was found for post-traumatic stress disorder. These observations require replication, however, they suggest that the CNR1 gene may be a risk factor for ADHD and possibly PTSD, and that this gene warrants further investigation for a role in neuropsychiatric disorders. |
SNPs reported by this study (count: 4)
SNP |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
rs806377 |
C/T |
|
P-value>0.05 |
showed no association with ADHD
showed no association with ADHD
|
Non-significant
|
rs6454674 |
G/T |
|
P-value>0.05 |
showed no association with ADHD
showed no association with ADHD
|
Non-significant
|
rs806368 |
C/T |
|
P-value>0.05 |
showed no association with ADHD
showed no association with ADHD
|
Non-significant
|
rs1049353 |
A/G |
|
P-value>0.05 |
showed no association with ADHD
showed no association with ADHD
|
Non-significant
|
Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
CNR1 |
haplotype CG P-value=0.008, significant association was dete......
haplotype CG P-value=0.008, significant association was detected with ADHD
More...
|
Significant
|